Regulation of Gene Expression and Epigenetics — Molecular Biology

Molecular Biology · Basic Sciences

How it begins

Estudio exhaustivo de los mecanismos moleculares que controlan cuándo, dónde y cuánto se expresa cada gen, desde los elementos cis-reguladores hasta las marcas epigenéticas heredables.

What it covers

1. Regulation of Gene Expression and Epigenetics
2. Introduction: the Central Dogma and the Levels of Regulation
3. Organization of Chromatin and the Nucleosome
4. · Chromatin fiber: compaction levels
5. · The nucleosome: the fundamental unit
6. Promoters, Cis-regulatory Elements and Promoter Architecture
7. · Structure of the eukaryotic promoter
8. Transcription Factors — Structure, Domains and Mechanisms
9. · Modular architecture of transcription factors
10. · Transcriptional activation and repression domains
11. Enhancers, Silencers, Insulators and 3D Genome Architecture
12. · Enhancers
13. · Silencers and Insulators
14. · Topological Association Domains (TADs)
15. Transcriptional Machinery: RNA Pol II, General Factors and Mediator
16. · The preinitiation complex (PIC)
17. · The CTD of RNA Pol II: phosphorylation code
18. · Promoter-proximal pausing — a critical regulatory step
19. · The Mediator Complex
20. Alternative Splicing — Proteome Diversification
21. · The spliceosome and the splicing mechanism
22. · Types of alternative splicing
23. · Splicing regulation: that, ESS, ISE, ISS elements
24. mRNA Stability — Degradation, NMD and ARE Elements
25. · Determinants of mRNA half-life
26. · Nonsense-Mediated mRNA Decay (NMD)
27. Translation Regulation — IRES, uORFs, eIF2α and mTOR
28. MicroRNA (miRNA) — Biogenesis, Mechanism and Regulatory Networks
29. · Canonical miRNA biogenesis
30. · Oncomiares and tumor suppressor miRNAs
31. Long Non-Coding RNA (lncRNA)
32. · Definition and characteristics
33. piRNA, siRNAEndogenous and Circular RNAs
34. DNA Methylation — DNMT, TET and Methylome Dynamics
35. · Cytosine methylation in CpG
36. · Active demethylation — TET enzymes and the 5hmC base
37. Post-translational Modifications of Histones
38. · The language of histone modifications
39. · HATs and HDACs — the acetylation/deacetylation balance
40. The Histone Code — Key Marks and Their Function
41. ATP-Dependent Chromatin Remodeling Complexes
42. Genomic Imprinting — Parental Monoallelism
43. · Definition and mechanism
44. X Chromosome Inactivation (XCI) — The Lyon Model
45. · The X inactivation process
46. Gene Regulation Networks — Circuit Logic
47. · Circuit motifs in gene networks
48. Epigenetics in Cancer — The Tumor Epigenome
49. · Global epigenetic alterations in cancer
50. Epigenetics in Development, Aging and Transgenerational Inheritance
51. · Epigenetic reprogramming in development
52. · The epigenetic clock and aging
53. Epigenetic Pharmacology — Approved Drugs and in Development