Molecular Biology: Clinical Applications

Molecular Biology: Clinical Applications — Expert Level

How it begins

Del laboratorio molecular a la cabecera del paciente: diagnóstico molecular, farmacogenómica, terapia génica, CRISPR, ARN terapéutico, oncología de precisión, inmuno-terapia molecular y medicina personalizada en profundidad extrema.

What it covers

1. Molecular Diagnostic Tools
2. · 1.1 PCR and its Diagnostic Variants
3. · 1.2 DNA Sequencing: From Sanger to the Third Generation
4. · 1.3 Molecular Cytogenomics
5. · 1.4 Complementary Hybridization Techniques
6. Liquid Biopsy
7. · 2.1 Circulating Tumor DNA (ctDNA)
8. · 2.2 Circulating Tumor Cells (CTCs)
9. · 2.3 Exosomes and Extracellular RNA
10. Pharmacogenomics
11. · 3.1 Polymorphisms in Drug Metabolism Enzymes (ADME)
12. · 3.2 Molecular Predictive Biomarkers in Oncology — Companion Diagnostics
13. Molecular Oncology: Targeted Therapies and Immunotherapy
14. · 4.1 Classes of Molecular Targets and their Inhibitors
15. · 4.2 Concept of Agnostic-Tumor (Pan-Tumor) Approvals
16. Gene Therapy: Vectors and Strategies
17. · 5.1 Viral Vectors
18. · 5.2 Non-Viral Vectors: Lipid Nanoparticles (LNPs)
19. · 5.3 Therapeutic Strategies
20. RNA-Based Therapies
21. · 6.1 Antisense Oligonucleotides (ASO)
22. · 6.2 Therapeutic Interference RNA (siRNA)
23. · 6.3 Therapeutic mRNA
24. CRISPR-Cas and Precision Gene Editing
25. · 7.1 Mechanism of CRISPR-Cas9
26. · 7.2 Variants of CRISPR Systems
27. · 7.3 CRISPR Therapies Approved and in Advanced Development
28. Molecular Diagnosis in Infectious Diseases
29. · 8.1 Multiplex PCR and Syndrome Diagnostic Panels
30. · 8.2 Molecular Diagnosis of Antimicrobial Resistance
31. · 8.3 Clinical Metagenomics (mNGS)
32. Clinical, Prenatal and Neonatal Genetics
33. · 9.1 Exome Sequencing in Pediatrics (Rapid WES)
34. · 9.2 Non-Invasive Prenatal Diagnosis (NIPT) — Free Fetal DNA in Plasma
35. · 9.3 Preimplantation Genetic Diagnosis (PGT)
36. · 9.4 Expanded Neonatal Screening (NBS)
37. Clinical Epigenomics and Epigenetic Drugs
38. · 10.1 Classifier of CNS Tumors by DNA Methylation
39. · 10.2 Approved Epigenetic Drugs
40. CAR-T and Adoptive Cellular Therapies
41. · 11.1 CAR-T Cells: Molecular Architecture and Manufacturing
42. · 11.2 CAR-T Approved — Current Overview
43. · 11.3 Characteristic Toxicities of CAR-T and Molecular Management
44. Clinical Bioinformatics and Artificial Intelligence in Genomics
45. · 12.1 Clinical NGS Bioinformatics Pipeline
46. · 12.2 Artificial Intelligence in Clinical Genomics
47. · 12.3 Interpretation of Variants According to ACMG/AMP Criteria