Flow Cytometry in Clinical Biochemistry — Atlas of Advanced Diagnostics

Biochemistry · Basic Sciences

How it begins

Análisis biofísico multiparamétrico de suspensiones celulares a nivel de célula única: fundamentos óptico-electrónicos, fluorocromos avanzados y aplicaciones diagnósticas de alta resolución

What it covers

1. Introduction and Fundamental Concepts
2. High Resolution Fluidics and Optics
3. · 1.1 Fluid Mechanics and Hydrodynamic Focusing
4. · 1.2 The Optical Excitation System
5. · 1.3 The Optical Light Collection and Dispersion System
6. · 1.4 Dichroic Mirrors, Optical Filters and Detectors
7. Electronic Processing and Biology of Fluorochromes
8. · 2.1 Electronic Signal Generation and Processing
9. · 2.2 Physics of Fluorescence and Stokes Shift
10. · 2.3 Families of Fluorochromes Used in Clinics
11. Spectral Compensation and Data Computing
12. · 3.1 The Spectral Superposition Problem (Spillover)
13. · 3.2 Mathematical Compensation of the Spill
14. · 3.3 Spectral Flow Cytometry and Spectral Unmixing
15. · 3.4 Gating Strategies and Advanced Computational Analysis
16. Vanguard Applications in Clinical Oncohematology
17. · 4.1 Diagnosis of Acute Leukemias and Lineage Assignment
18. · 4.2 Diagnosis of Chronic Lymphoproliferative Syndromes (SLPC-B)
19. · 4.3 Diagnosis and Staging of Multiple Myeloma (MM)
20. Clinical Immunology and Immune Monitoring
21. · 5.1 Monitoring of T, B and NK Subpopulations (TBNK Panel)
22. · 5.2 Primary Immunodeficiencies (PID)
23. · 5.3 Functional Tests: Oxidative Burst (Dihydrorhodamine 123)
24. · 5.4 HLA-B27 typing
25. Other High Complexity Biochemical and Clinical Applications
26. · 6.1 Analysis of Cell Cycle and DNA Content (Ploidy)
27. · 6.2 Detection of Apoptosis using Annexin V and Propidium Iodide
28. · 6.3 Cytometry of Extracellular Vesicles (Microparticles)
29. · 6.4 Determination of CD34+ Hematopoietic Progenitors (ISHAGE Protocol)
30. Quality Control, Standardization and Analytical Validation
31. · 7.1 Daily Instrument Calibration and Instrument to Instrument
32. · 7.2 Standardization of International Consortia (EuroFlow)
33. · 7.3 Critical Preanalytical Variables in Biochemistry and Flow Hematology
34. · 7.4 Synthesis of Clinical Applications by Pathology
35. · Clinical-Biochemical Integrating Principle