Clinical Biochemistry: Stem Cells and Regenerative Medicine

Biochemistry · Basic Sciences

How it begins

Estudio bioquímico y molecular exhaustivo de los sistemas de células madre. Análisis detallado de los mecanismos epigenéticos de autorrenovación, reprogramación por factores de Yamanaka, reprogramación química, plasticidad metabólica, microambiente tumoral y aplicaciones de vanguardia en medicina regenerativa.

What it covers

1. Physiology of Power, Self-renewal and Plasticity
2. · Molecular Hierarchy of Power
3. Biochemistry of the Stem Cell Niche and Metabolic Plasticity
4. · Biochemical and Biophysical Components of the Niche
5. · Metabolic Plasticity: Glycolysis vs. Oxidative Phosphorylation
6. Genomic Circuit of Pluripotency and Transcription Factors
7. · Mechanisms of Action of Core Factors
8. · Auxiliary Factors of the Pluripotency Network
9. Epigenetic Regulation and Chromatin Architecture
10. · Histone Marks and Bivalent Domains
11. · Biochemistry of DNA Methylation
12. Intracellular Signaling and Signal Transduction Pathways
13. · Main Signaling Pathways in Stem Cells
14. Biochemistry of Cellular Reprogramming (iPSCs)
15. · Temporal Phases of Somatic Reprogramming
16. · Chemical Reprogramming: Small Molecules as Substitutes for Factors
17. Embryonic Stem Cells (ESCs): Naive and Primed States
18. Biochemistry of Hematopoietic Stem Cells (HSCs)
19. · Structure of the Bone Marrow Niche
20. · The CXCL12/CXCR4 Axis and HSC Homing
21. Mesenchymal or Stromal Stem Cells (MSCs)
22. · Definition Criteria of MSCs according to the ISCT
23. · Biochemistry of Immunomodulation by MSCs
24. Biochemistry of Neural Stem Cells (NSCs)
25. · Cell Structure and Lineage in the Dentate Gyrus
26. Epithelial and Intestinal Stem Cells (Lgr5+)
27. · The Intestinal Crypt Niche and Lgr5+ Cells
28. · Epidermal Stem Cells (Bulge / Protuberance of the Hair Follicle)
29. Cancer Stem Cells (CSCs) or Tumors
30. · Surface Biomarkers of CSCs by Cancer Types
31. · Biochemistry of Chemo and Radioresistance in CSCs
32. Biochemistry of Senescence and Aging of Stem Cells
33. · The Cascade of Replicative and Oncogenic Senescence
34. · The Senescence-Associated Secretory Phenotype (SASP)
35. · Biology of Telomeres in Stem Cells
36. Organoids and Molecular Modeling of Diseases
37. · Stages of Differentiation in the Modeling of Brain Organoids
38. Cell Therapy and Advanced Regenerative Medicine
39. · Immunological Barriers and the Development of Hypoallergenic Stem Cells
40. Cellular Biosafety, Karyotype and Instability and Teratoma Assays
41. · The Teratoma Test: Gold Test for Pluripotency
42. · Karyotypic Instability and Clonal Enrichment in Vitro
43. Bioethical Governance, Health Regulation and GMP Standards
44. · Fundamental Lines of Bioethical Regulation
45. · Clinical Grade Manufacturing Standards (GMP - Good Manufacturing Practices)