Clinical Biochemistry of microRNAs | Treatise on Genomic Medicine and Molecular Diagnostics

Biochemistry · Basic Sciences

How it begins

Estudio detallado de la biogénesis, mecanismos efectores y dinámicas extracelulares de los pequeños RNAs no codificantes de cadena sencilla (~19-24 nucleótidos). Un análisis exhaustivo de su papel regulador sobre la traducción del mensajero, su estabilidad en fluidos corporales y su traslación clínica como biomarcadores diagnósticos, pronósticos y agentes terapéuticos de última generación.

What it covers

1. Clinical biochemistry of microRNAs
2. Biogenesis of microRNAs: Canonical and Non-Canonical Pathways
3. · 1.1 · Nuclear Transcription: From pri-miRNA to pre-miRNA
4. · 1.2 · Nucleocytoplasmic Export and Processing by Dicer
5. · 1.3 · Strand Selection and Assembly of the RISC Complex
6. · 1.4 · Non-Canonical Biogenesis Pathways
7. Molecular Mechanisms of Gene Silencing
8. · 2.1 · Types of Mating and Thermodynamic Rules
9. · 2.2 · Translational Repression vs. mRNA degradation
10. · 2.3 · Cellular Compartmentalization: Processing Bodies (P-bodies)
11. Stability, Secretion and Extracellular Transport of microRNAs
12. · 3.1 · Stability and Physical Protection Mechanisms
13. · 3.2 · Active Cellular Secretion Routes
14. · 3.3 · Paracrine and Endocrine Signaling: Functionality in Recipient Cells
15. Quantification, Isolation and Normalization Methodologies
16. · 4.1 · Isolation and Purification Strategies of RNA Fractions
17. · 4.2 · Quantification Technological Platforms
18. · 4.3 · Methodological Normalization Strategies
19. Oncological Biomarkers I: Solid Tumors
20. · 5.1 · Epigenetic Signatures in Breast Cancer: The miR-21 and miR-10b Cascades
21. · 5.2 · MicroRNA Signatures in Lung and Colon Adenocarcinoma
22. Onco-miRs in Malignant Hematology: Leukemias and Lymphomas
23. · 6.1 · Chronic Lymphatic Leukemia (CLL): The 13q14 Deletion and the miR-15a/16-1 Cluster
24. · 6.2 · Diffuse Large B-Cell Lymphoma (DLBCL) and the miR-155 Signature
25. Molecular Cardiology, MyomiRs and Acute Coronary Syndromes
26. · 7.1 · Molecular Kinetics of MyomiRs Release in Acute Myocardial Infarction
27. · 7.2 · Pathophysiology of Ventricular Remodeling and Cardiac Fibrosis
28. Neurology and Neurodegenerative Diseases: Alzheimer's and Parkinson's
29. · 8.1 · Alzheimer's Disease: Deregulation of the miR-29a/b-1 and BACE1 Axis
30. · 8.2 · Parkinson's Disease: The Role of miR-133b and Dopamine Homeostasis
31. Endocrinology, Diabetes Mellitus and Metabolic Disorders
32. · 9.1 · Pathophysiology of the Pancreatic Beta Cell: The master role of miR-375
33. · 9.2 · microRNAs in Metabolic Hepatology: Hepatic steatosis and the role of miR-122
34. Nephrology and Kidney Diseases: Diabetic Nephropathy and AKI
35. · 10.1 · Diabetic Nephropathy: The miR-192 and miR-21 Cascades induced by TGF-β1
36. · 10.2 · Early Diagnosis of Acute Kidney Injury (AKI)
37. Hepatology and Viral Interactions: Hepatitis B, C and NASH
38. · 11.1 · Structural Dependence of the Hepatitis C Virus (HCV)
39. · 11.2 · microRNAs in Metabolic Steatohepatitis (NASH / MASH)
40. Immunology, Autoimmunity and Chronic Inflammation
41. · 12.1 · microRNAs in Autoimmunity: The Cascades of Systemic Lupus Erythematosus and Rheumatoid Arthritis
42. · 12.2 · The Concept of "Inflammaging" and microRNAs
43. Obstetrics, Gynecology and Maternal-Fetal Medicine
44. · 13.1 · Pathophysiology of Preeclampsia: The Master Role of miR-210
45. · 13.2 · The C19MC Cluster as a Biomarker of Fetal Well-being
46. Forensic Medicine and Toxicogenomics
47. · 14.1 · Identification of Biological Fluids at the Crime Scene
48. · 14.2 · Applications in Toxicogenomics and Early Detection of Organic Damage
49. Therapeutics Based on microRNAs: Mimics and Inhibitors
50. · 15.1 · microRNA Mimics: Chemical Stabilization and Delivery Vectors
51. · 15.2 · Inhibition Strategies: AntagomiRs and Target Site Blockers
52. · 15.3 · Next Generation Clinical Trials: Successes and Technical Limitations
53. Bioinformatics, Target Prediction and Pharmacogenomics
54. · 16.1 · Molecular Target Prediction Algorithms
55. · 16.2 · Pharmacogenomics and miRSNPs
56. · 16.3 · Key Principles in the Practice of Clinical Biochemistry of microRNAs